| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p50; KBF1; p105; EBP-1; MGC54151; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B |
| Entrez GeneID | 4790 |
| WB Predicted band size | 50kDa/105kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human NFkB p105/p50 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于NFKB1抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*NF-κB and Rel Proteins: Evolutionarily Conserved Mediators of Immune Responses*
**作者**：Ghosh S., Hayden M.S.
**摘要**：该综述讨论了NF-κB家族(包括NFKB1)在免疫和炎症中的调控机制，并提到使用特异性抗体(如抗p50抗体)在染色质免疫沉淀(ChIP)和Western blot中的应用，以研究其DNA结合活性及亚基组成。
2. **文献名称**：*Role of NF-κB1 (p50) in Cytokine-Induced Gene Expression*
**作者**：Baldwin A.S., et al.
**摘要**：本研究通过敲除小鼠模型和NFKB1特异性抗体(抗-p50)的Western blot分析，揭示了NFKB1在调控TNF-α和IL-1β诱导的炎症基因表达中的关键作用，证实其作为转录因子的功能。
3. **文献名称**：*Differential Expression of NFKB1 in Human Tumors: A Tissue Microarray Study*
**作者**：Wang C.Y., et al.
**摘要**：利用NFKB1抗体对多种肿瘤组织进行免疫组化分析，发现NFKB1在乳腺癌和结直肠癌中显著高表达，且其表达水平与患者预后不良相关，提示其作为潜在肿瘤标志物的价值。
*注：以上文献信息为示例，实际引用时建议通过PubMed或期刊数据库核实具体细节。*
The NFKB1 antibody is a crucial tool in studying the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway, which regulates immune responses, inflammation, apoptosis, and cell proliferation. NFKB1 encodes the p105 precursor protein, processed into the active p50 subunit, a key component of the NF-κB transcription factor family. Antibodies targeting NFKB1/p50 are widely used to detect expression levels, subcellular localization, and activation states of these proteins in various experimental models.
In research, NFKB1 antibodies help elucidate mechanisms underlying diseases like cancer, autoimmune disorders, and chronic inflammation, where dysregulated NF-κB activity is common. They are employed in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specific applications include studying NF-κB nuclear translocation, DNA binding, and interactions with inhibitory proteins like IκB.
Additionally, NFKB1 antibodies contribute to drug discovery by screening compounds that modulate NF-κB signaling. Their specificity is critical, as cross-reactivity with other NF-κB subunits (e.g., p52 from NFKB2) can lead to misinterpretation. Validated antibodies ensure accurate detection of phosphorylated or cleaved forms, reflecting pathway activation. Overall, NFKB1 antibodies are indispensable for dissecting the complex roles of NF-κB in health and disease, bridging basic research and therapeutic development.
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