首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人尿皮素-2(UCN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的UCN2含量。UCN2 是一个靶点。UCN2 即尿皮质素 2，属促肾上腺皮质激素释放因子家族，参与调节应激反应、心血管功能及能量代谢。研究机制上，其通过与特定受体结合激活下游信号通路，为相关疾病如心血管病、代谢病的治疗带来新方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于体外研究场景，如探究UCN2在代谢调控相关细胞模型中的分泌动态、动物实验中的组织分布分析，或临床前研究中的生物标志物探索，为心血管疾病、肥胖症及应激相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SRP ELISA Kit; Stresscopin related peptide ELISA Kit; Stresscopin-related peptide ELISA Kit; Ucn II ELISA Kit; Ucn2 ELISA Kit; UCN2_HUMAN ELISA Kit; UCNI ELISA Kit; UCNII ELISA Kit; UR ELISA Kit; Urocortin II ELISA Kit; Urocortin related peptide ELISA Kit; Urocortin-2 ELISA Kit; Urocortin-related peptide ELISA Kit; URP ELISA Kit
缩写：UCN2
Uniprot No.：Q96RP3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human UCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human UCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 104 | 100-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.2582.3022.2802.1422001.7351.6961.7161.5781001.1261.1011.1140.976500.6780.6370.6580.520250.4120.4340.4230.28512.50.3110.3090.3100.1726.250.2430.2540.2490.11100.1370.1390.138 | ||||||
货期：3-5 working days
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