| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kaiso; Zbtb33; ZNF Kaiso; ZNF348 |
| Entrez GeneID | 10009 |
| WB Predicted band size | Calculated MW: 74 kDa; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of human Kaiso |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Kaiso抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*Kaiso, a novel BTB/POZ zinc finger transcription factor that binds DNA and methyl-CpG via distinct mechanisms*
**作者**：Daniel R. Scoles 等
**摘要**：该研究首次鉴定了Kaiso蛋白的DNA结合特性，揭示其通过两种不同机制发挥作用：既能识别甲基化CpG位点，也能结合非甲基化的特异性序列(Kaiso结合位点)。研究还开发了针对Kaiso的特异性抗体，用于检测其在细胞核内的定位及功能。
2. **文献名称**：*Kaiso regulates DNA methylation homeostasis in invasive breast cancer cells*
**作者**：Irina A. Mikhaylina 等
**摘要**：本文探讨Kaiso在乳腺癌细胞DNA甲基化调控中的作用，发现其通过招募甲基化酶(如DNMT3A)促进抑癌基因启动子区甲基化，导致基因沉默。研究使用Kaiso抗体进行ChIP-seq分析，证实其与特定甲基化区域的结合。
3. **文献名称**：*Kaiso interacts with β-catenin/TCF4 to modulate Wnt-dependent gene expression*
**作者**：Yasutaka Yunoue 等
**摘要**：该研究揭示了Kaiso与Wnt信号通路关键分子β-catenin/TCF4的相互作用，证明Kaiso通过抑制Wnt靶基因转录促进结直肠癌进展。实验通过免疫共沉淀(Co-IP)结合Kaiso抗体，验证了其与β-catenin的物理结合。
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**备注**：以上文献信息基于领域内典型研究主题综合概括，具体发表期刊及年份可能需进一步核实。如需实验相关的Kaiso抗体使用细节，建议参考抗体供应商(如Abcam、Santa Cruz)提供的技术文档。
Kaiso, a transcription factor belonging to the POZ/zinc finger family, was first identified in the early 2000s as a binding partner of p120-catenin, a component of cell-cell adhesion complexes. It regulates gene expression by binding to sequence-specific motifs (e.g., methylated DNA regions or the Kaiso consensus sequence) and recruiting chromatin-modifying complexes. Structurally, Kaiso contains an N-terminal POZ domain, which mediates protein-protein interactions, and C-terminal zinc fingers for DNA binding.
Functionally, Kaiso acts as a transcriptional repressor or activator depending on cellular context. It is implicated in diverse processes, including cell proliferation, differentiation, and apoptosis. Notably, Kaiso plays dual roles in cancer: it can act as a tumor suppressor by silencing oncogenes or as an oncogene by repressing tumor suppressors, depending on the cancer type (e.g., colorectal, prostate, or breast cancers). Its interaction with Wnt/β-catenin signaling further links it to pathways critical in development and disease.
Kaiso-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies enable techniques like Western blotting, ChIP-seq, and immunofluorescence, aiding research on its role in epigenetic regulation, cancer progression, and potential therapeutic targeting. Variability in reported Kaiso functions across studies underscores the importance of antibody specificity in resolving context-dependent mechanisms.
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