首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠泛素羧基末端水解酶L1(UCHL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的UCHL1含量。大鼠泛素羧基末端水解酶L1（UCHL1）在多种生理和病理过程中发挥重要作用，包括神经元功能、细胞周期调控和泛素化过程。其表达与细胞状态和代谢活性相关，并在多种疾病如神经退行性疾病和炎症反应中扮演关键角色。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品操作流程标准化，可在4.5小时内完成检测，为科研人员研究泛素 - 蛋白酶体系统功能、神经退行性病变分子机制、药物干预效果评估等提供可靠工具，尤其适用于体外模型构建、疾病标志物筛选及分子通路调控等基础研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Uchl1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; UCH-L1 ELISA Kit; EC 3.4.19.12 ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit
缩写：UCHL1
Uniprot No.：Q00981
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat UCHL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 98-106 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 100-108 | |||||
回收率：
| The recovery of rat UCHL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-90 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.1962.1742.1852.050251.6181.6351.6271.49212.51.0711.0981.0850.9506.250.6820.6540.6680.5333.120.4500.4360.4430.3081.560.3560.3680.3620.2270.780.2510.2650.2580.12300.1340.1360.135 | ||||||
货期：3-5 working days
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