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产品描述：人扭曲原肠胚蛋白同源物1(TWSG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TWSG1含量。TWSG1是一种与BMP信号通路相关的蛋白，在胚胎发育和组织稳态中起重要作用。它通过形成三元复合物与CHRD和BMP结合，既充当拮抗剂又充当激动剂，影响BMP信号传导。TWSG1在软骨发育、细胞分化等过程中发挥关键作用，其功能研究有助于理解相关疾病的发生机制。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于基础科研领域，如探索TWSG1在胚胎发育调控、骨骼疾病机制、肿瘤微环境调控或代谢相关信号通路中的功能，也可用于评估疾病模型中TWSG1的表达水平变化，为相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PSEC0250 ELISA Kit; twisted gastrulation homolog 1 (Drosophila) ELISA Kit; TSG ELISA Kit; TSG1 ELISA Kit; Twisted gastrulation BMP signaling modulator 1 ELISA Kit; Twisted gastrulation homolog 1 ELISA Kit; Twisted gastrulation protein ELISA Kit; Twisted gastrulation protein homolog 1 ELISA Kit; TWSG1 ELISA Kit; TWSG1_HUMAN ELISA Kit
缩写：TWSG1
Uniprot No.：Q9GZX9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TWSG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human TWSG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.5572.7652.6612.54240001.8161.9021.8591.74020001.1031.1891.1461.02710000.7070.7140.7110.5925000.4050.3740.3900.2712500.3120.3090.3110.1921250.2050.2010.2030.08400.1230.1150.119 | ||||||
货期：3-5 working days
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