首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转甲状腺素蛋白(TTR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TTR含量。TTR即甲状腺素转运蛋白，它在人体内参与甲状腺素和视黄醇的转运。研究发现TTR异常聚集形成淀粉样纤维会导致多种疾病。其研究机制主要围绕抑制TTR聚集、稳定TTR结构等方面，以开发治疗相关疾病的药物。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于体外实验中对小鼠TTR的定量分析，可广泛用于基础医学研究中疾病机制探索、基因工程动物模型的表型验证、药物干预效果评价等场景，为神经科学、代谢疾病及蛋白质错误折叠相关领域提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ttr ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit
缩写：TTR
Uniprot No.：P07309
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:8000 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of mouse TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.2852.1842.2352.061 1001.6921.8131.7531.579 501.3351.3561.3461.172 250.9130.9240.9190.745 12.50.6510.6400.6460.472 6.250.4120.4010.4070.233 3.120.3120.3070.3100.136 00.1740.1730.174 | ||||||
货期：3-5 working days
×
