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产品描述：人微管蛋白β3(TUBB3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TUBB3含量。TUBB3 属于微管蛋白β-Ⅲ类，在神经发育等过程中发挥重要作用。研究显示它与肿瘤发生发展、耐药密切相关。其机制在于它影响微管动力学稳定性，改变肿瘤细胞的迁移、侵袭能力，还会干扰化疗药物与微管结合，降低疗效。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于神经科学领域的基础研究，包括神经元分化模型评估、化疗药物对微管稳定性影响的机制探索，以及脑组织或神经母细胞瘤细胞系中TUBB3表达谱分析，为研究微管相关细胞活动提供可靠的定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta 3 tubulin ELISA Kit; beta 4 ELISA Kit; beta-4 ELISA Kit; CDCBM ELISA Kit; CDCBM1 ELISA Kit; CFEOM3 ELISA Kit; CFEOM3A ELISA Kit; FEOM3 ELISA Kit; M(beta)3 ELISA Kit; M(beta)6 ELISA Kit; MC1R ELISA Kit; Neuron specific beta III Tubulin ELISA Kit; Neuron-specific class III beta-tubulin ELISA Kit; QccE-11995 ELISA Kit; QccE-15186 ELISA Kit; TBB3_HUMAN ELISA Kit; Tubb 3 ELISA Kit; TUBB3 ELISA Kit; TUBB4 ELISA Kit; Tubulin beta 3 ELISA Kit; Tubulin beta 3 chain ELISA Kit; Tubulin beta 4 ELISA Kit; Tubulin beta III ELISA Kit; Tubulin beta-3 chain ELISA Kit; Tubulin beta-4 chain ELISA Kit; Tubulin beta-III ELISA Kit; tuj 1 ELISA Kit; tuj1 ELISA Kit
缩写：TUBB3
Uniprot No.：Q13509
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TUBB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 81-93 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human TUBB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 96-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.3522.3842.3682.264201.8491.8691.8591.755101.1281.1671.1481.04450.5830.5420.5630.4592.50.3550.3360.3460.2421.250.2440.2370.2410.1370.6250.1810.1720.1770.07300.1060.1020.104 | ||||||
货期：3-5 working days
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