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产品描述：人三结构域包含蛋白72(TRIM72)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TRIM72含量。TRIM72是一种三结构域蛋白，参与细胞膜修复和抗氧化功能，可能通过膜修复、抗氧化和线粒体功能修复等机制减缓神经退行性疾病如肌萎缩侧索硬化症（ALS）的神经元损害。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于探究TRIM72在肌肉损伤修复、再生医学或疾病模型（如心肌缺血、骨骼肌病变等）中的分子机制研究；可满足细胞生物学、分子病理学等领域的基础科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LOC493829 ELISA Kit; Mg53 ELISA Kit; Mitsugumin-53 ELISA Kit; TRI72_HUMAN ELISA Kit; trim72 ELISA Kit; Tripartite motif containing 72 ELISA Kit; Tripartite motif containing protein 72 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit
缩写：TRIM72
Uniprot No.：Q6ZMU5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of human TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 103 | 98-107 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.5082.3102.4092.2848001.4421.3451.3941.2694000.7790.8160.7980.6732000.4630.4460.4550.3301000.3220.3170.3200.195500.2390.2310.2350.110250.1980.1920.1950.07000.1260.1240.125 | ||||||
货期：3-5 working days
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