首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠促甲状腺素释放激素(TRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRH含量。TRH即促甲状腺激素释放激素，它由下丘脑合成并释放，能刺激腺垂体分泌促甲状腺激素。在研究机制上，其与腺垂体细胞膜上受体结合，通过特定信号通路发挥作用，对调节甲状腺功能至关重要，在甲状腺相关疾病等研究领域受广泛关注。试剂盒检测范围为0.5 pg/mL-100 pg/mL，特别适合用于研究下丘脑 - 垂体 - 甲状腺轴调控机制，例如在甲状腺功能异常、代谢紊乱或神经退行性疾病动物模型中评估 TRH 的动态变化，也可用于药理学研究中的药物干预效果分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
缩写：TRH
Uniprot No.：P01150
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.5 pg/mL-100 pg/mL
灵敏度：0.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 96-104 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 97-105 | |||||
回收率：
| The recovery of rat TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 1000.1500.1460.148 250.3010.2980.300 80.4870.5000.494 20.7760.7900.783 0.51.2641.2881.276 01.9321.9071.920 | ||||||
货期：3-5 working days
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