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产品描述：人E3泛素蛋白连接酶TRIM63(TRIM63)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TRIM63含量。TRIM63也称MuRF1，是肌肉特异性泛素连接酶。其背景在于与肌肉萎缩密切相关。研究机制方面，它能识别并泛素化特定底物蛋白，促使其通过蛋白酶体途径降解，在肌肉蛋白稳态维持、肌肉萎缩调控等方面发挥重要作用。试剂盒检测范围为12.5 pg/mL-800 pg/mL，该产品适用于肌肉生物学、运动医学及代谢性疾病等基础研究领域，可探索TRIM63在骨骼肌再生、心肌肥大或肿瘤微环境中的功能机制，为体外模型、动物实验或人类组织样本的蛋白表达分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：E3 ubiquitin-protein ligase TRIM63 ELISA Kit; FLJ32380 ELISA Kit; IRF ELISA Kit; Iris RING finger protein ELISA Kit; MURF 1 ELISA Kit; MURF-1 ELISA Kit; MuRF1 ELISA Kit; MURF2 ELISA Kit; Muscle specific ring finger protein 1 ELISA Kit; Muscle specific ring finger protein 2 ELISA Kit; Muscle-specific RING finger protein 1 ELISA Kit; OTTHUMP00000008701 ELISA Kit; RING finger protein 28 ELISA Kit; RNF 28 ELISA Kit; RNF28 ELISA Kit; SMRZ ELISA Kit; Striated muscle RING zinc finger protein ELISA Kit; TRI63_HUMAN ELISA Kit; TRIM 63 ELISA Kit; Trim63 ELISA Kit; Tripartite motif containing 63 ELISA Kit; tripartite motif containing 63, E3 ubiquitin protein ligase ELISA Kit; Tripartite motif containing protein 63 ELISA Kit; Tripartite motif-containing protein 63 ELISA Kit; Ubiquitin ligase TRIM63 ELISA Kit
缩写：TRIM63
Uniprot No.：Q969Q1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.1 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:20 | Average % | 102 | ||||
| Range % | 99-104 | |||||
| 1:40 | Average % | 88 | ||||
| Range % | 86-90 | |||||
回收率：
| The recovery of human TRIM63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.5082.7252.6172.4304002.0132.1122.0631.8762001.3371.3251.3311.1441000.8250.8270.8260.639500.4740.4400.4570.270250.3390.3300.3350.14812.50.2710.2820.2770.09000.1840.1890.187 | ||||||
货期：3-5 working days
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