首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠促甲状腺素释放激素(TRH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清样本中的TRH含量。TRH即促甲状腺激素释放激素，是下丘脑分泌的神经激素。其主要作用为刺激腺垂体分泌促甲状腺激素（TSH），进而调节甲状腺功能。研究机制上，TRH与腺垂体细胞表面受体结合，通过特定信号通路发挥生理效应，在甲状腺轴调控等方面意义重大。试剂盒检测范围为2.5 pg/mL-160 pg/mL，适用于探索小鼠甲状腺功能相关疾病模型、神经内分泌调控机制、药物干预效果评估等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Trh ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
缩写：TRH
Uniprot No.：Q62361
种属：Mus musculus (Mouse)
样本类型：serum
检测范围：2.5 pg/mL-160 pg/mL
灵敏度：0.625 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 91-113 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
回收率：
| The recovery of mouse TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected1602.6042.4752.5402.421801.4821.4081.4451.326400.9170.9690.9430.824200.6210.6430.6320.513100.3750.3860.3810.26250.2960.2810.2890.1702.50.1680.1720.1700.05100.1210.1170.119 | |||||||
货期：3-5 working days
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