首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肌钙蛋白I，骨骼肌慢肌(TNNI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNNI1含量。TNNI1是心肌肌钙蛋白I的慢骨骼肌亚型，主要表达于慢肌纤维。作为心肌重要调节蛋白，它参与肌肉收缩舒张的调控。当前研究聚焦其在心肌病等疾病中表达变化及机制，以探索其作为潜在诊断标志物和治疗靶点的可能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于多种科研场景，如骨骼肌损伤模型的生物标志物分析、肌肉代谢相关研究、运动医学中肌纤维类型转换机制探索，以及药理学实验中药物对慢肌功能影响的评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnni1 ELISA Kit; Troponin I ELISA Kit; slow skeletal muscle ELISA Kit; Troponin I ELISA Kit; slow-twitch isoform ELISA Kit
缩写：TNNI1
Uniprot No.：P13413
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of rat TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 84-92 | |||||
| EDTA plasma (n=4) | 103 | 99-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.5262.4232.4752.289 5001.8751.7561.8161.630 2501.2121.1591.1861.000 1250.7840.8110.7980.612 62.50.5000.4900.4950.309 31.20.3470.3620.3550.169 15.60.2910.2740.2830.097 00.1830.1880.186 | |||||||
货期：3-5 working days
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