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产品描述：人肌钙蛋白I，骨骼肌慢肌(TNNI1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TNNI1含量。TNNI1 即心肌肌钙蛋白 I1，是心肌肌钙蛋白复合物的调节亚基。研究聚焦于其在心肌收缩舒张调控中的作用机制。异常表达或修饰可能影响心肌功能，它可作为心肌疾病如心肌病等潜在诊断和治疗靶点，为相关疾病干预开辟新思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对骨骼肌功能机制、运动相关代谢研究、肌肉退行性疾病模型构建及药物干预效果评估等场景，为探索TNNI1在肌肉生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2700018B22Rik ELISA Kit; AI747285 ELISA Kit; DKFZp451O223 ELISA Kit; si:dz182n13.3 ELISA Kit; slow skeletal muscle ELISA Kit; slow-twitch isoform ELISA Kit; SSTNI ELISA Kit; TNN1 ELISA Kit; Tnni1 ELISA Kit; TNNI1_HUMAN ELISA Kit; Troponin I ELISA Kit; Troponin I slow skeletal muscle ELISA Kit; Troponin I slow twitch isoform ELISA Kit; troponin I type 1 (skeletal; slow) ELISA Kit; Troponin I; slow isoform ELISA Kit
缩写：TNNI1
Uniprot No.：P19237
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.7432.7682.7562.615101.9191.9091.9141.77351.0561.0871.0720.9312.50.5840.5800.5820.4411.250.3570.3870.3720.2310.6250.2830.2990.2910.1500.3120.1940.1980.1960.05500.1420.1400.141 | ||||||
货期：3-5 working days
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