| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 1/50-1/100 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | DAPK2; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1 |
| Entrez GeneID | 23604 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | A synthetic peptide of human DAPK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于DAPK2抗体的3篇参考文献示例(部分信息为模拟概括，仅供参考)：
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1. **文献名称**：*DAPK2 regulates oxidative stress in apoptosis and autophagy via p38MAPK signaling*
**作者**：Chen L, et al.
**摘要**：研究利用DAPK2特异性抗体，通过Western blot和免疫荧光技术，证明DAPK2在氧化应激条件下通过激活p38MAPK通路调控细胞凋亡与自噬，为DAPK2在肿瘤治疗中的潜在作用提供机制依据。
2. **文献名称**：*Death-associated protein kinase 2 modulates T cell receptor signaling and T cell activation*
**作者**：Schumacher AM, et al.
**摘要**：通过DAPK2抗体阻断实验，发现DAPK2负向调控T细胞受体(TCR)信号通路，抑制T细胞活化与增殖，提示其在自身免疫性疾病中的潜在治疗靶点价值。
3. **文献名称**：*DAPK2 expression correlates with chemoresistance in acute myeloid leukemia*
**作者**：Ramos JM, et al.
**摘要**：采用免疫组化及流式细胞术结合DAPK2抗体，发现DAPK2高表达与急性髓系白血病(AML)患者对化疗耐药性相关，其机制可能通过抑制线粒体凋亡通路实现。
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**注意**：以上文献信息为示例性概括，实际文献需通过PubMed、Google Scholar等平台以“DAPK2 antibody”或“DAPK2 function”为关键词检索获取。如需具体文献，建议提供研究方向(如疾病模型、信号通路等)以便进一步筛选。
**Background of DAPK2 Antibody**
Death-associated protein kinase 2 (DAPK2) is a calcium/calmodulin-regulated serine/threonine kinase belonging to the DAPK family, which includes five members involved in apoptosis, autophagy, and stress responses. DAPK2 shares structural homology with DAPK1. featuring a kinase domain, a calmodulin-regulatory domain, and a death domain. Unlike DAPK1. DAPK2 lacks ankyrin repeats and is primarily localized in the cytoplasm, though it can translocate to the nucleus under specific conditions.
DAPK2 plays a critical role in regulating programmed cell death, particularly in hematopoietic cells. It is implicated in erythroid differentiation, autophagy-mediated cell death, and suppression of tumor progression. Dysregulation of DAPK2 has been linked to cancers (e.g., leukemia, lymphoma), autoimmune disorders, and inflammatory diseases. For instance, reduced DAPK2 expression is associated with poor prognosis in certain malignancies, while its overexpression induces apoptosis and inhibits metastasis.
DAPK2 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous DAPK2 in cell lines and tissues. These antibodies are often validated using knockout controls or siRNA-mediated silencing to ensure specificity. Monoclonal and polyclonal variants are available, targeting epitopes within the kinase or regulatory domains. Research applications span cancer biology, neurobiology (due to links with neurodegenerative pathways), and immunology, particularly in understanding DAPK2's role in immune cell regulation. Emerging studies also explore DAPK2 as a therapeutic target, driving demand for high-quality antibodies in drug development.
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