关键信息
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基因名
IkBe
- 应用
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别名
NFKBIE; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Epsilon
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O54910
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表达区间
Ala115~Phe350
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分子量
32kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
IkB proteins play a crucial role in the regulation of the NF-κB signaling pathway, which is pivotal in controlling immune responses, inflammation, and cell survival. Upon stimulation by various cytokines or stressors, IkB proteins bind to NF-κB dimers, preventing their nuclear translocation and subsequent transcription of target genes. The phosphorylation and subsequent degradation of IkB proteins, usually mediated by IKK (IkB kinase), release NF-κB, allowing it to enter the nucleus and exert its biological effects. Given their central role in numerous diseases, including cancer, autoimmune disorders, and chronic inflammatory conditions, the study of recombinant IkB proteins has gained significant attention. Researchers are investigating the design and application of these recombinant proteins to better understand NF-κB regulation and its implications in pathology. Additionally, recombinant IkB proteins offer potential therapeutic avenues, as they could be utilized to inhibit NF-κB activity in disease states, thereby mitigating inflammation or tumor progression. This research aims to uncover novel strategies for targeting the NF-κB pathway, with the ultimate goal of developing new treatments for diseases linked to its dysregulation.
