Mouse Tumor necrosis factor ligand superfamily member 9(TNFSF9) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL023997MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠肿瘤坏死因子配体超家族成员9(TNFSF9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF9含量。TNFSF9 即肿瘤坏死因子超家族成员 9，也叫 4-1BBL ，是一种重要的免疫靶点。它可与 4-1BB 结合激活下游信号通路，增强 T 细胞、NK 细胞活性和增殖能力，在肿瘤免疫治疗领域是研究热点，有望开发出更有效的抗癌疗法。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本试剂盒适用于自身免疫性疾病模型、肿瘤免疫治疗机制研究、小鼠感染性疾病模型、肿瘤移植模型或药物干预实验中的分子水平动态监测，可用于免疫通路研究、药物靶点验证及病理机制探索，支持科研人员高效开展体外样本分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tnfsf9; Cd137l; Cd157l; Ly63l; Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL
缩写：
TNFSF9
Uniprot No.：
P41274
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
23.5 pg/mL-1500 pg/mL
灵敏度：
5.8 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFSF9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	96
	Range %	89-103
1:4	Average %	89
	Range %	84-95
1:8	Average %	92
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1500	2.049	2.134	2.091	1.968
750	1.452	1.389	1.420	1.297
375	0.900	0.928	0.914	0.790
187.5	0.571	0.609	0.590	0.466
94	0.330	0.321	0.326	0.202
47	0.231	0.243	0.237	0.113
23.5	0.178	0.184	0.181	0.058
0	0.122	0.125	0.124