| WB | 咨询技术 | Human,Rat,Hamster |
| IF | 咨询技术 | Human,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Rat,Hamster |
| ICC | 技术咨询 | Human,Rat,Hamster |
| FCM | 咨询技术 | Human,Rat,Hamster |
| Elisa | 咨询技术 | Human,Rat,Hamster |
| Aliases | CUL4B; KIAA0695; Cullin-4B; CUL-4B |
| Entrez GeneID | 8450 |
| WB Predicted band size | Calculated MW: 104 kDa; Observed MW: 88,104 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Hamster |
| Immunogen | A synthetic peptide of human Cullin 4B |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cullin 4A/4B抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: *Cullin 4A/B Antibodies Reveal Distinct Roles in DNA Damage Response*
**作者**: Li, X. et al.
**摘要**: 本研究利用特异性Cullin 4A和4B抗体，通过免疫沉淀和免疫荧光技术，揭示了Cullin 4A在调控CRL4复合物介导的DNA损伤修复通路中的独特功能，而Cullin 4B更多参与细胞周期调控。
2. **文献名称**: *Characterization of Cullin 4B Antibody for Detecting Tumor-Specific Expression*
**作者**: Wang, Y. et al.
**摘要**: 开发并验证了一种高特异性Cullin 4B单克隆抗体，证实其在多种癌症组织中高表达，且与患者预后不良相关，为癌症诊断提供了潜在标志物。
3. **文献名称**: *Cullin 4A Antibody Identifies Its Interaction with Viral Oncoproteins*
**作者**: Gupta, R. et al.
**摘要**: 通过Cullin 4A抗体筛选发现其与人乳头瘤病毒(HPV)E7蛋白结合，揭示病毒通过劫持CRL4A复合物促进宿主细胞泛素化降解的分子机制。
(注：以上文献为示例，实际引用请查询PubMed等数据库获取真实研究。)
Cullin 4A (CUL4A) and Cullin 4B (CUL4B) are core scaffold proteins of the Cullin-RING ligase 4 (CRL4) family, a subclass of ubiquitin E3 ligases that regulate protein degradation via the ubiquitin-proteasome system. Structurally, both share a conserved cullin homology domain and three tandem repeats critical for binding the adaptor protein DDB1. which recruits substrate receptors (e.g., DDB2. CSA, or WDR proteins) to form functional CRL4 complexes. CUL4A and CUL4B are paralogs with ~80% sequence identity but differ in their N-terminal regions; CUL4B contains an extended N-terminal nuclear localization signal.
CRL4 complexes mediate diverse cellular processes, including DNA repair, replication, chromatin remodeling, and cell cycle regulation. CUL4A is implicated in nucleotide excision repair (e.g., UV-induced DNA damage via DDB2), while CUL4B associates with epigenetic regulators like histone methyltransferases, influencing transcription and development. Dysregulation of CUL4A/B is linked to diseases: CUL4A overexpression correlates with cancers (e.g., breast, lung), whereas CUL4B mutations cause X-linked intellectual disability syndromes.
Antibodies targeting CUL4A/B are essential tools for studying their expression, localization, and molecular interactions (e.g., via Western blot, immunoprecipitation). Due to high homology, antibody specificity is critical; most are designed against divergent regions (e.g., C-terminal sequences). These antibodies aid in exploring CRL4’s role in disease mechanisms and potential therapeutic targeting.
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