Human Tumor necrosis factor ligand superfamily member 14(TNFSF14) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL023991HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人肿瘤坏死因子配体超家族成员14(TNFSF14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的TNFSF14含量。TNFSF14 属于肿瘤坏死因子超家族成员。它在免疫调节、肿瘤发生发展等过程中发挥作用。研究机制方面，其能与受体结合，激活下游信号通路，参与调节细胞存活、增殖和免疫反应等，有可能成为肿瘤治疗等领域的潜在靶点。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，适用于基础科研领域中对TNFSF14蛋白表达量动态变化的精准分析，例如免疫细胞功能研究、肿瘤微环境分子机制探索或炎症相关动物模型实验的样本检测，可为免疫学、分子病理学及疾病机制研究提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
TNFSF14; HVEML; LIGHT; UNQ391/PRO726; Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; CD antigen CD258
缩写：
TNFSF14
Uniprot No.：
O43557
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
0.156 ng/ml - 10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TNFSF14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	102
	Range %	94-107
1:4	Average %	95
	Range %	89-99
1:8	Average %	92
	Range %	88-96

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.902	2.844	2.873	2.758
5	2.007	2.167	2.087	1.972
2.5	1.218	1.246	1.232	1.117
1.25	0.664	0.676	0.670	0.555
0.625	0.397	0.384	0.391	0.276
0.312	0.243	0.258	0.251	0.136
0.156	0.165	0.169	0.167	0.052
0	0.114	0.115	0.115