首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B细胞活化因子（BAFF）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF13B含量。TNFSF13B，也称为BAFF，是一种激活B细胞的细胞因子，属于肿瘤坏死因子家族。它参与B细胞的成熟和存活，与自身免疫性疾病如系统性红斑狼疮和类风湿关节炎有关，其研究机制涉及与受体结合，激活信号传导途径，促进B细胞增殖和分化。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研领域可应用于探索BAFF在红斑狼疮、干燥综合征等自身免疫疾病中的调控机制，或用于评估药物干预后B细胞活化通路的变化。针对不同样本类型，实验者可灵活设计研究方案，如通过血清/血浆检测循环系统中BAFF的动态变化，或利用组织匀浆分析特定器官的局部表达特征，为免疫学及疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ApoL related ligand TALL 1 ELISA Kit; B cell Activating Factor ELISA Kit; B lymphocyte stimulator ELISA Kit; B-cell-activating factor ELISA Kit; BAFF ELISA Kit; BLyS ELISA Kit; CD 257 ELISA Kit; CD257 ELISA Kit; CD257 antigen ELISA Kit; Delta BAFF ELISA Kit; Dendritic cell derived TNF like molecule ELISA Kit; Dendritic cell-derived TNF-like molecule ELISA Kit; DTL ELISA Kit; DTL precursor ELISA Kit; PRO738 ELISA Kit; soluble form ELISA Kit; TALL 1 ELISA Kit; TALL-1 ELISA Kit; TALL1 ELISA Kit; THANK ELISA Kit; TN13B_HUMAN ELISA Kit; TNF and APOL related leukocyte expressed ligand 1 ELISA Kit; TNF homolog that activates apoptosis ELISA Kit; TNF homolog that activates apoptosis NKFB and JNK. ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 1 ELISA Kit; TNFSF13B ELISA Kit; TNFSF20 ELISA Kit; TNLG7A ELISA Kit; Tumor necrosis factor (ligand) superfamily member 13b ELISA Kit; Tumor necrosis factor ligand 7A ELISA Kit; Tumor necrosis factor ligand superfamily member 13b ELISA Kit; Tumor necrosis factor ligand superfamily member 20 ELISA Kit; Tumor necrosis factor like protein ZTNF4 ELISA Kit; Tumor necrosis factor superfamily member 13B ELISA Kit; UNQ401 ELISA Kit; ZTNF4 ELISA Kit
缩写：TNFSF13B
Uniprot No.：Q9Y275
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BAFF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of human BAFF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 90 | 87-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.9122.9532.9332.819102.1422.1752.1592.04551.2951.3121.3041.1902.50.7270.6950.7110.5971.250.4260.4430.4350.3210.6250.2970.3120.3050.1910.3120.2470.2610.2540.14000.1120.1150.114 | ||||||
货期：3-5 working days
×
