首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子配体超家族成员13(TNFSF13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的TNFSF13含量。TNFSF13即肿瘤坏死因子配体超家族成员13，在免疫调节、细胞生长等过程起重要作用。其异常表达与多种疾病相关，如自身免疫病、肿瘤等。研究机制主要聚焦于其信号通路，明确它如何与受体结合，影响下游信号传导，为疾病治疗提供靶点思路。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于体外实验中对小鼠模型中TNFSF13表达水平的动态监测，如免疫系统功能研究、炎症相关疾病模型评估或药物干预效果分析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfsf13 ELISA Kit; April ELISA Kit; Tumor necrosis factor ligand superfamily member 13 ELISA Kit; A proliferation-inducing ligand ELISA Kit; APRIL ELISA Kit; CD antigen CD256 ELISA Kit
缩写：TNFSF13
Uniprot No.：Q9D777
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.43 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFSF13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of mouse TNFSF13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 100 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.6362.4962.5662.45530002.1232.0892.1061.99515001.4771.4561.4671.3567500.7760.7530.7650.6543750.3720.3660.3690.258187.50.2660.2540.2600.14993.750.1940.1870.1910.08000.1120.1100.111 | ||||||
货期：3-5 working days
×
