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产品描述:人增殖诱导配体(APRIL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、尿液样本中的TNFSF13含量。TNFSF13属于肿瘤坏死因子配体超家族,在免疫系统中发挥关键作用。对其研究机制主要聚焦在它与免疫细胞的相互作用,以及在疾病如自身免疫病、癌症中的表达异常。深入了解TNFSF13有望为相关疾病的诊断和治疗提供新靶点与策略。试剂盒检测范围为0.78 ng/mL-50 ng/mL,适用于免疫调控机制研究、疾病模型生物标志物分析、药物干预效果评估等科研场景,尤其适用于比较不同病理条件下 APRIL 在循环系统或泌尿系统中的表达差异,为探索其与 BAFF 受体信号通路的相互作用提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:A proliferation inducing ligand ELISA Kit; A proliferation-inducing ligand ELISA Kit; APRIL ELISA Kit; CD256 ELISA Kit; TALL-2 ELISA Kit; TALL2 ELISA Kit; TNF related death ligand ELISA Kit; TNF- and APOL-related leukocyte expressed ligand 2 ELISA Kit; TNF-related death ligand 1 ELISA Kit; TNF13_HUMAN ELISA Kit; TNFSF13 ELISA Kit; TRDL-1 ELISA Kit; TRDL1 ELISA Kit; Tumor necrosis factor (ligand) superfamily; member 13 ELISA Kit; Tumor necrosis factor ligand superfamily member 13 ELISA Kit; Tumor necrosis factor like protein ZTNF2 ELISA Kit; Tumor necrosis factor related death ligand 1 ELISA Kit; UNQ383/PRO715 ELISA Kit; ZTNF2 ELISA Kit
缩写:TNFSF13
Uniprot No.:O75888
种属:Homo sapiens (Human)
样本类型:serum, plasma, urine
检测范围:0.78 ng/mL-50 ng/mL
灵敏度:0.195 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human APRIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 94 | ||||
Range % | 90-98 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-97 | |||||
1:4 | Average % | 86 | ||||
Range % | 82-90 | |||||
1:8 | Average % | 98 | ||||
Range % | 94-102 |
回收率:
The recovery of human APRIL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 104 | 100-108 | ||||
EDTA plasma (n=4) | 97 | 92-101 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 502.2852.3522.3192.179 251.6051.5651.5851.445 12.51.0531.1041.0790.939 6.250.6560.6790.6680.528 3.120.4110.3960.4040.264 1.560.2980.3180.3080.168 0.780.2120.2090.2110.071 00.1430.1370.140 |
货期:3-5 working days
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