Mouse Tumor necrosis factor ligand superfamily member 12(TNFSF12) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL023987MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠肿瘤坏死因子配体超家族成员12(TNFSF12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFSF12含量。TNFSF12是肿瘤坏死因子超家族成员，在细胞增殖、分化及凋亡等生理病理过程起关键作用。研究发现它可通过与特定受体结合，激活相关信号通路，参与炎症反应、肿瘤发生发展等。深入探究其机制，有助于开发相关疾病治疗新策略。试剂盒检测范围为78 pg/ml- 5000 pg/ml，适用于体外实验中对细胞因子动态变化的定量研究，例如免疫系统功能评估、炎症相关疾病模型分析或肿瘤微环境中分子调控机制的探索。适用于基础医学、免疫学及分子生物学等领域的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tnfsf12; Tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; TWEAK
缩写：
TNFSF12
Uniprot No.：
O54907
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml - 5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFSF12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	86-98
1:2	Average %	100
	Range %	90-104
1:4	Average %	94
	Range %	85-97
1:8	Average %	98
	Range %	91-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.720	2.657	2.689	2.568
2500	2.017	1.996	2.007	1.886
1250	1.379	1.412	1.396	1.275
625	0.798	0.767	0.783	0.662
312	0.489	0.498	0.494	0.373
156	0.268	0.261	0.265	0.144
78	0.192	0.185	0.189	0.068
0	0.120	0.122	0.121