| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PTGS1; COX1; Prostaglandin G/H synthase 1; Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1 |
| Entrez GeneID | 5742 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human COX1/Cyclooxygenase 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Cyclooxygenase 1(COX-1)抗体的3篇代表性文献的简要信息：
1. **文献名称**：*"Immunohistochemical localization of cyclooxygenase-1 in human tissues"*
**作者**：Smith, W.L. et al.
**摘要**：该研究利用COX-1特异性抗体，通过免疫组化技术系统分析了COX-1在人类多种正常组织(如胃、肾、血管内皮)中的分布，证实其在维持基础生理功能中的作用，如胃黏膜保护和血小板功能调节。
2. **文献名称**：*"Selectivity of nonsteroidal anti-inflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase"*
**作者**：Mitchell, J.A., Warner, T.D., & FitzGerald, G.A.
**摘要**：文章比较了不同NSAIDs对COX-1和COX-2的抑制活性，并通过抗体验证了COX-1在血小板和胃肠道中的高表达，强调COX-1抗体在评估药物靶向特异性中的重要性。
3. **文献名称**：*"Characterization of monoclonal antibodies against cyclooxygenase-1 (COX-1) and their application in COX-1-deficient mouse models"*
**作者**：Sheng, H. et al.
**摘要**：研究开发了高特异性抗COX-1单克隆抗体，并通过Western blot和免疫荧光验证其在野生型与COX-1敲除小鼠组织中的反应性，为COX-1功能研究提供可靠工具。
4. **文献名称**：*"Cyclooxygenase-1-dependent prostaglandins in cerebral microvascular endothelial cells during infection"*
**作者**：Candelario-Jalil, E. et al.
**摘要**：利用COX-1抗体探究了脑微血管内皮细胞中COX-1的炎症调控作用，发现其在感染模型中通过PGE2生成影响血脑屏障功能，提示COX-1在神经炎症中的潜在治疗价值。
这些文献涵盖了抗体开发、组织分布、功能验证及疾病模型应用，可作为COX-1相关研究的参考。
Cyclooxygenase 1 (COX-1) is a constitutively expressed enzyme that plays a key role in maintaining physiological functions, such as gastric mucosal protection, platelet aggregation, and renal homeostasis. It catalyzes the conversion of arachidonic acid to prostaglandin H2. a precursor for various prostanoids. Unlike its inducible isoform COX-2. which is associated with inflammation and pathology, COX-1 is ubiquitously present in most tissues under normal conditions.
Antibodies targeting COX-1 are essential tools for studying its expression, localization, and regulatory mechanisms in both healthy and diseased states. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences derived from specific regions of the COX-1 protein, such as the catalytic domain or C-terminus. Validation methods, including knockout cell lines or tissue samples, ensure specificity by confirming reduced signal in COX-1-deficient models.
COX-1 antibodies are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate tissue-specific expression patterns. They also aid in exploring COX-1's role in diseases, such as gastrointestinal disorders (e.g., peptic ulcers) or cardiovascular conditions linked to altered prostaglandin synthesis. Additionally, these antibodies help evaluate the selectivity of COX inhibitors (e.g., aspirin) by distinguishing COX-1 activity from COX-2. critical for drug development and safety profiling.
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