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Human Soluble Receptor Activator of Nuclear Factor-kB,sRANK ELISA Kit

  • 中文名： 人可溶性核因子κB受体活化因子(sRANK)ELISA Kit
货号: IPD-E13539h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人可溶性核因子κB受体活化因子(sRANK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNFRSF11A含量。TNFRSF11A是一个重要靶点。它是肿瘤坏死因子受体超家族成员，在骨代谢、免疫调节等生理过程中发挥关键作用。其研究机制主要聚焦于它与配体结合激活信号通路，参与破骨细胞生成调控等，是相关疾病药物研发的潜在靶点。试剂盒检测范围为0.312 ng/ml-20 ng/ml.，灵敏度为0.078 ng/ml.。适用于基础科研中骨代谢调控机制研究、炎症性疾病模型分析、肿瘤微环境探索等领域，尤其可为骨相关疾病生物标志物筛选、药物干预效果评估提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CD 265 ELISA Kit; CD265 ELISA Kit; FEO ELISA Kit; LOH18CR1 ELISA Kit; Loss of heterozygosity 18 chromosomal region 1 ELISA Kit; mRANK ELISA Kit; ODFR ELISA Kit; OFE ELISA Kit; OPTB7 ELISA Kit; Osteoclast differentiation factor receptor ELISA Kit; OSTS ELISA Kit; Paget disease of bone 2 ELISA Kit; PDB 2 ELISA Kit; PDB2 ELISA Kit; RANK ELISA Kit; Receptor activator of NF KB ELISA Kit; Receptor activator of NF-KB ELISA Kit; receptor activator of nuclear factor kappa B ELISA Kit; TNF receptor superfamily member 11a ELISA Kit; TNFRSF11A ELISA Kit; TNR11_HUMAN ELISA Kit; TRANCER ELISA Kit; Tumor necrosis factor receptor superfamily member 11a NFKB activator ELISA Kit; Tumor necrosis factor receptor superfamily member 11a activator of NFKB ELISA Kit; Tumor necrosis factor receptor superfamily member 11A ELISA Kit
  • 缩写：
    TNFRSF11A
  • Uniprot No.：
    Q9Y6Q6
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.3125 ng/ml- 20ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human sRANK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    86-94


    1:2

    Average %

    101


    Range %

    94-104


    1:4

    Average %

    91


    Range %

    85-94


    1:8

    Average %

    95


    Range %

    90-98


  • 回收率：

    The recovery of human sRANK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    84-94


    EDTA plasma (n=4)

    95

    89-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    1.981

    2.012

    1.997

    1.898


    10

    1.523

    1.514

    1.519

    1.420


    5

    0.886

    0.867

    0.877

    0.778


    2.5

    0.425

    0.445

    0.435

    0.336


    1.25

    0.290

    0.303

    0.297

    0.198


    0.625

    0.215

    0.222

    0.219

    0.120


    0.312

    0.154

    0.157

    0.156

    0.057


    0

    0.100

    0.098

    0.099




  • 货期：
    3-5 working days

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