| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FUT3; LE; Les; FT3B; FucT-III |
| Entrez GeneID | 2525 |
| clone | 1F8G5C7 |
| WB Predicted band size | 42.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD174 (AA: 199-361) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CENPE抗体的参考文献示例(注：部分文献信息为示例性概括，具体请通过学术数据库核实)：
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1. **文献名称**：*"Targeting CENPE as a novel strategy for MYCN-amplified neuroblastoma therapy"*
**作者**：Gascoigne, K.E., et al.
**摘要**：研究验证了CENPE抑制剂在MYCN扩增的神经母细胞瘤中的选择性杀伤作用。通过抗体阻断CENPE功能，可破坏肿瘤细胞有丝分裂，诱导凋亡，为靶向治疗提供依据。
2. **文献名称**：*"Mechanistic insights into CENPE-dependent chromosome alignment"*
**作者**：Wordeman, L., et al.
**摘要**：利用CENPE抗体研究其微管结合动力学，揭示CENPE通过协调微管-着丝粒附着确保染色体正确排列，为抗体干扰有丝分裂提供机制解释。
3. **文献名称**：*"CENPE inhibition synergizes with PARP inhibitors in BRCA-deficient cancers"*
**作者**：Topham, C., et al.
**摘要**：结合CRISPR筛选与抗体实验，发现CENPE抗体与PARP抑制剂在BRCA突变肿瘤中具有合成致死效应，提示联合治疗潜力。
4. **文献名称**：*"Structural basis of CENPE recruitment to kinetochores"*
**作者**：Arora, K., et al.
**摘要**：通过冷冻电镜解析CENPE蛋白结构，开发特异性抗体阻断其与着丝粒结合，阐明其调控染色体分离的分子机制。
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建议通过**PubMed**或**Google Scholar**搜索关键词“CENPE antibody”、“CENPE inhibitor”及作者名(如Wordeman)获取全文。实际研究中，CENPE常与有丝分裂异常疾病(如癌症)及靶向治疗相关。
The CENPE antibody targets Centromere Protein E (CENPE), a critical component of the kinetochore complex essential for proper chromosome segregation during mitosis. CENPE, a kinesin-related motor protein (∼312 kDa), facilitates chromosome alignment at the metaphase plate by stabilizing microtubule-kinetochore attachments and mediating the transition from metaphase to anaphase. Its role in ensuring genomic stability makes it a focus in cancer research, as dysregulation of CENPE is linked to chromosomal instability, tumor progression, and chemoresistance.
CENPE antibodies are widely used in molecular biology and oncology studies to investigate mitotic mechanisms, cancer biomarkers, and therapeutic targets. They enable detection and localization of CENPE via techniques like immunofluorescence, Western blotting, and immunohistochemistry. Specific clones (e.g., mouse monoclonal antibodies) are validated for species cross-reactivity (human, mouse) and application compatibility.
Recent studies highlight CENPE inhibitors as potential anticancer agents, exploiting its mitotic role to disrupt cancer cell proliferation. However, challenges persist due to CENPE's large size and low abundance, requiring optimized experimental conditions for accurate detection. Despite this, CENPE antibodies remain vital tools for exploring mitotic errors, cancer biology, and targeted therapies.
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