首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肿瘤坏死因子诱导基因蛋白6(TNFAIP6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TNFAIP6含量。TNFAIP6 是一种重要靶点。它在多种生理和病理过程中发挥作用，如炎症调节等。研究机制方面，其可能通过参与蛋白聚糖形成、稳定细胞外基质等方式影响细胞行为和信号传导，在疾病治疗等领域有潜在研究价值。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中探究炎症性疾病模型（如关节炎）、创伤修复机制、肿瘤微环境调控等领域的分子机理研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tnfaip6 ELISA kit; Tnfip6 ELISA kit; Tsg6Tumor necrosis factor-inducible gene 6 protein ELISA kit; TNF-stimulated gene 6 protein ELISA kit; TSG-6 ELISA kit; Tumor necrosis factor alpha-induced protein 6 ELISA kit; TNF alpha-induced protein 6 ELISA kit
缩写：TNFAIP6
Uniprot No.：O08859
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFAIP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of mouse TNFAIP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-95 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5962.4522.5242.33420002.1362.1732.1551.96510001.7391.7041.7221.5325001.2381.1791.2091.0192500.7070.7610.7340.5441250.4280.4020.4150.22562.50.2590.2770.2680.07800.1930.1870.190 | ||||||
货期：3-5 working days
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