首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肿瘤坏死因子相关凋亡诱导配体受体2(TRAIL-R2/DR5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TNFRSF10B含量。TNFRSF10B是重要的靶点。它属于肿瘤坏死因子受体超家族，在细胞凋亡调控等过程中发挥关键作用。其研究机制主要聚焦于与配体结合后激活下游信号通路，影响细胞增殖、存活等，为肿瘤等疾病治疗提供潜在靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于基础科研中细胞凋亡机制研究、肿瘤微环境分析、抗癌药物筛选及疗效评估等领域，尤其适合通过血清或血浆样本监测实验模型中TRAIL - R2/DR5的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TNFRSF10B; DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; UNQ160/PRO186; Tumor necrosis factor receptor superfamily member 10B; Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; TRAIL-R2; CD antigen CD262
缩写：TNFRSF10B
Uniprot No.：O14763
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL-R2/DR5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 81-89 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 87-94 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
回收率：
| The recovery of human TRAIL-R2/DR5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.6042.5982.6012.514 10002.2932.2042.2492.162 5001.6871.6581.6731.586 2501.0871.0021.0450.958 1250.6290.6380.6340.547 62.50.4540.4680.4610.374 31.250.2470.2310.2390.152 00.0850.0890.087 | |||||||
货期：3-5 working days
×
