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Rabbit Monoclonal Catalase Antibody

  • 中文名: Catalase抗体
  • 别    名: Cas1; CAT; Catalase; Cs1
货号: IPDX21167
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCas1; CAT; Catalase; Cs1
Entrez GeneID847
WB Predicted band sizeCalculated MW: 60 kDa; Observed MW: 60 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human Catalase
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于Catalase抗体的代表性文献摘要(基于公开研究内容概括,非真实文献):

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1. **文献名称**:*Development and Characterization of a Novel Monoclonal Antibody Against Human Catalase*

**作者**:Smith et al.

**摘要**:该研究开发了一种针对人类过氧化氢酶(Catalase)的高特异性单克隆抗体,通过Western blot和免疫荧光验证了其在多种细胞系中的结合能力,并证明其可用于检测Catalase的亚细胞定位(如过氧化物酶体)。

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2. **文献名称**:*Catalase Expression in Neurodegenerative Disorders: An Immunohistochemical Study Using Catalase-Specific Antibodies*

**作者**:Lee & Tanaka

**摘要**:利用Catalase特异性抗体,通过免疫组化分析阿尔茨海默病和帕金森病患者脑组织,发现Catalase表达水平与氧化应激损伤程度呈负相关,提示其在神经退行性疾病中的保护作用。

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3. **文献名称**:*A Comparative Study of Catalase Activity Assays: Validation of Antibody-Based Detection Methods*

**作者**:García-Ruiz et al.

**摘要**:比较了多种Catalase活性检测方法,包括基于抗体的ELISA和化学法。研究发现抗体法在低浓度样本中灵敏度更高,但需结合活性测定以避免交叉反应干扰。

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**注**:以上为模拟摘要,实际文献需通过PubMed或Google Scholar检索关键词(如"Catalase antibody"、"Catalase immunohistochemistry")获取。

背景信息

Catalase antibodies are immunological tools designed to detect and study the enzyme catalase, a critical antioxidant protein found in nearly all aerobic organisms. Catalase plays a vital role in cellular defense by catalyzing the decomposition of hydrogen peroxide (H₂O₂) into water and oxygen, thereby protecting cells from oxidative damage. Dysregulation of catalase activity is linked to various pathologies, including neurodegenerative diseases, cancer, diabetes, and aging-related disorders. Researchers employ catalase antibodies in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to investigate catalase expression, localization, and function in biological samples. These antibodies are often produced in hosts such as rabbits or mice, using purified catalase proteins or peptide fragments as immunogens. They exhibit specificity for catalase across species, including human, mouse, and rat, and are validated for applications in tissue sections, cell lysates, and cultured cells. Catalase antibodies are essential in studying oxidative stress mechanisms, evaluating therapeutic interventions targeting reactive oxygen species (ROS), and exploring catalase's role in disease models. Quality control often involves knockout validation to ensure minimal cross-reactivity with unrelated proteins. Their utility spans basic research, diagnostic development, and drug discovery.

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