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产品描述：人蛋白-谷氨酰胺γ-谷氨酰转移酶4(TGM4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TGM4含量。试剂盒检测范围为50 pg/mL-3200 pg/mL，支持科研人员开展前列腺功能调控、生殖系统疾病机制探索或肿瘤微环境相关研究，为体外实验提供稳定可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Dorsal prostate transglutaminase ELISA Kit; Dorsal protein 1 ELISA Kit; Dp1 ELISA Kit; Fibrinoligase ELISA Kit; hTGP ELISA Kit; Prostate specific transglutaminase ELISA Kit; Prostate transglutaminase ELISA Kit; Prostate-specific transglutaminase ELISA Kit; Protein glutamine gamma glutamyltransferase 4 ELISA Kit; Protein-glutamine gamma-glutamyltransferase 4 ELISA Kit; TG(P) ELISA Kit; TGase P ELISA Kit; TGase-4 ELISA Kit; TGase4 ELISA Kit; Tgm4 ELISA Kit; TGM4_HUMAN ELISA Kit; TGP ELISA Kit; Transglutaminase 4 (prostate) ELISA Kit; Transglutaminase 4 ELISA Kit; Transglutaminase P ELISA Kit; Transglutaminase, prostate ELISA Kit; Transglutaminase-4 ELISA Kit
缩写：TGM4
Uniprot No.：P49221
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：50 pg/mL-3200 pg/mL
灵敏度：12.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 81-97 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 83-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-110 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
回收率：
| The recovery of human TGM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-104 | |||||
| EDTA plasma (n=4) | 93 | 88-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected32002.7242.7052.7152.62216002.0582.0172.0381.9458001.3821.3691.3761.2834000.9070.9240.9160.8232000.5810.5840.5830.4901000.3310.3390.3350.242500.2220.2200.2210.12800.0910.0950.093 | |||||||
货期：3-5 working days
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