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产品描述：人蛋白谷氨酰胺γ谷氨酰转移酶K(TGM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TGM1含量。TGM1 即转谷氨酰胺酶 1，它是一种膜结合酶。在TGM1 功能缺陷与常染色体隐性遗传性鱼鳞病相关。研究机制上，它主要参与角质形成细胞包膜的交联形成，对皮肤屏障功能的维持至关重要，目前其相关研究有助于此类皮肤病治疗策略的开发。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于基础医学研究中皮肤屏障功能调控机制探索、细胞分化模型建立、疾病相关生物标志物筛选以及药物开发过程中功效评价等科研场景，为研究表皮生物学、遗传性皮肤病发病机理及治疗策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ARCI1 ELISA Kit; Epidermal TGase ELISA Kit; ICR2 ELISA Kit; KTG ELISA Kit; LI ELISA Kit; LI1 ELISA Kit; Protein glutamine gamma glutamyltransferase K ELISA Kit; Protein-glutamine gamma-glutamyltransferase K ELISA Kit; TG(K) ELISA Kit; TGase 1 ELISA Kit; TGASE ELISA Kit; TGase K ELISA Kit; TGase-1 ELISA Kit; TGK ELISA Kit; TGM1 ELISA Kit; TGM1_HUMAN ELISA Kit; Transglutaminase 1 (K polypeptide epidermal type I; protein glutamine gamma glutamyltransferase) ELISA Kit; Transglutaminase 1 ELISA Kit; Transglutaminase K ELISA Kit; Transglutaminase; keratinocyte ELISA Kit; Transglutaminase-1 ELISA Kit
缩写：TGM1
Uniprot No.：P22735
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 84-90 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 108 | |||||
| Range % | 104-112 | ||||||
| 1:8 | Average % | 83 | |||||
| Range % | 80-86 | ||||||
回收率：
| The recovery of human TGM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-96 | |||||
| EDTA plasma (n=4) | 100 | 97-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 16002.2152.3012.2582.145 8001.7181.7021.7101.597 4001.1371.2041.1711.058 2000.8020.8310.8170.704 1000.4990.4890.4940.381 500.3350.3420.3390.226 250.2130.2310.2220.109 00.1120.1140.113 | |||||||
货期：3-5 working days
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