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产品描述：人转谷氨酰胺酶2C多肽(TGM2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGM2含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研领域，如探究TGM2在疾病发生发展中的分子机制、评估药物干预对TGM2活性的调节作用，或通过动物模型及体外实验分析不同组织或体液样本中TGM2的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALPHA SUBUNIT ELISA Kit; C polypeptide ELISA Kit; EC 2.3.2.13 ELISA Kit; epididymis secretory protein Li 45 ELISA Kit; G alpha h ELISA Kit; G[a]h ELISA Kit; Gh CLASS G ALPHA h ELISA Kit; GNAH ELISA Kit; GNAH G PROTEIN ELISA Kit; H POLYPEPTIDE ELISA Kit; HEL-S-45 ELISA Kit; Protein glutamine gamma glutamyltransferase 2 ELISA Kit; Protein-glutamine gamma-glutamyltransferase 2 ELISA Kit; TG 2 ELISA Kit; TG(C) ELISA Kit; TG2 ELISA Kit; TGase C ELISA Kit; TGase H ELISA Kit; TGase-2 ELISA Kit; TgaseII ELISA Kit; TGC ELISA Kit; TGC GUANINE NUCLEOTIDE BINDING PROTEIN ELISA Kit; TGM2 ELISA Kit; TGM2_HUMAN ELISA Kit; Tissue transglutaminase ELISA Kit; Transglutaminase 2 ELISA Kit; Transglutaminase 2 C polypeptide ELISA Kit; Transglutaminase C ELISA Kit; Transglutaminase H ELISA Kit; Transglutaminase-2 ELISA Kit; tTG ELISA Kit; tTGas ELISA Kit
缩写：TGM2
Uniprot No.：P21980
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TGM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 86 | ||||
| Range % | 81-91 | |||||
| 1:10 | Average % | 99 | ||||
| Range % | 93-102 | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-95 | |||||
回收率：
| The recovery of human TGM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 97 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.7362.6542.6952.5636002.4132.3212.3672.2353001.9081.8541.8811.7491500.9670.9880.9780.846750.6980.7020.7000.56837.50.3880.4130.4010.26918.750.2770.2640.2710.13900.1340.1290.132 | ||||||
货期：3-5 working days
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