首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠甲状腺球蛋白(TG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TG含量。试剂盒检测范围为10 ng/mL-160 ng/mL，适用于甲状腺疾病模型研究、自身免疫性甲状腺炎机制探索、甲状腺代谢调控实验等科研场景，为甲状腺相关分子机制研究及药物干预效果评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tg ELISA Kit; Tgn ELISA Kit; Thyroglobulin ELISA Kit; Tg ELISA Kit
缩写：TG
Uniprot No.：O08710
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：10 ng/mL-160 ng/mL
灵敏度：10 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-100 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 82-100 | ||||||
| 1:4 | Average % | 93 | |||||
| Range % | 88-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-105 | ||||||
回收率：
| The recovery of mouse TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-110 | |||||
| EDTA plasma (n=4) | 95 | 89-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 1600.1650.1700.168 800.2760.2710.274 400.5040.4990.502 201.0081.0341.021 102.0552.0292.042 | |||||||
货期：3-5 working days
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