| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx |
| Entrez GeneID | 22809 |
| WB Predicted band size | Calculated MW: 31 kDa; Observed MW: 31 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ATF5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与ATF5抗体相关的代表性文献(摘要为简化概括)：
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1. **文献名称**: *ATF5 regulates the proliferation and survival of breast cancer cells by modulating the unfolded protein response*
**作者**: Wang Q, et al.
**摘要**: 本研究利用ATF5特异性抗体，通过免疫印迹和免疫组化发现ATF5在乳腺癌中高表达，并通过调控未折叠蛋白反应(UPR)促进肿瘤细胞存活，提示其作为潜在治疗靶点。
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2. **文献名称**: *Activating Transcription Factor 5 (ATF5) is required for neural stem cell proliferation and self-renewal*
**作者**: Angelastro JM, et al.
**摘要**: 通过ATF5抗体染色与基因敲除实验，证实ATF5在小鼠神经干细胞中高表达，并调控其增殖与自我更新能力，抑制ATF5导致干细胞分化加速。
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3. **文献名称**: *Targeting ATF5 in glioblastoma using a novel class of protein phosphatase 2A-activating drugs*
**作者**: Sheng Z, et al.
**摘要**: 使用ATF5抗体进行免疫沉淀和ChIP-seq分析，揭示胶质母细胞瘤中ATF5通过激活PP2A磷酸酶抑制肿瘤生长，为药物开发提供新策略。
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如需具体DOI或期刊信息，可进一步补充实验背景或应用场景。
ATF5 (Activating Transcription Factor 5) is a member of the ATF/CREB family of transcription factors, which regulate gene expression in response to cellular stress, proliferation, differentiation, and apoptosis. It plays a critical role in maintaining cellular homeostasis, particularly in secretory cells, neurons, and cancer biology. ATF5 is implicated in tumorigenesis by promoting cell survival under stress, and its dysregulation is associated with cancers such as glioblastoma, breast cancer, and leukemia.
ATF5 antibodies are essential tools for detecting and quantifying ATF5 protein levels, localization, and activity in research. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). They are typically developed in various host species (e.g., rabbit, mouse) and validated for specificity using knockout controls or peptide competition assays.
Commercial ATF5 antibodies are available from multiple suppliers (e.g., CST, Abcam, Santa Cruz Biotechnology), often targeting conserved regions like the N-terminal domain. Researchers prioritize antibodies with high specificity and low cross-reactivity to related family members (e.g., ATF4). Validation in specific experimental contexts is critical due to variable post-translational modifications or tissue-specific isoforms. ATF5 antibodies have advanced studies in cancer mechanisms, neurobiology, and stem cell regulation, offering insights into therapeutic targeting pathways.
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