首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转铁蛋白受体(TFR/CD71)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TFRC含量。TFRC即转铁蛋白受体，是细胞摄取铁元素的主要载体，在细胞生长和增殖中起重要作用。研究发现，它在多种肿瘤细胞中高表达。其机制在于它能促进肿瘤细胞的铁摄取，维持肿瘤细胞的生长和存活，成为肿瘤诊断与治疗的潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于评估铁代谢异常模型、造血系统疾病机制研究及抗肿瘤药物干预实验中细胞增殖状态的动态监测，助力铁离子相关信号通路及细胞功能调控的机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tfrc ELISA Kit; Trfr ELISA Kit; Transferrin receptor protein 1 ELISA Kit; TR ELISA Kit; TfR ELISA Kit; TfR1 ELISA Kit; Trfr ELISA Kit; CD antigen CD71 ELISA Kit
缩写：TFRC
Uniprot No.：Q62351
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 82-99 | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 85-102 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 83-100 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 89-108 | |||||
回收率：
| The recovery of mouse TFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-104 | ||||
| EDTA plasma (n=4) | 97 | 85-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.0041.9391.9721.809 201.5481.5141.5311.368 101.1061.0551.0810.918 50.6730.6450.6590.496 2.50.4420.4210.4320.269 1.250.3020.2880.2950.132 0.6250.2270.2180.2230.060 00.1610.1650.163 | ||||||
货期：3-5 working days
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