| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR |
| Entrez GeneID | 196 |
| WB Predicted band size | Calculated MW: 96 kDa; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Aryl hydrocarbon Receptor |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Aryl Hydrocarbon Receptor(AhR)抗体的3篇参考文献概览：
1. **"Aryl hydrocarbon receptor regulates apoptosis and inflammation in a murine model of experimental autoimmune uveitis"**
*作者：Zhang Y, et al.*
**摘要**：研究利用AhR特异性抗体检测AhR在小鼠自身免疫性葡萄膜炎模型中的表达，发现AhR通过调控炎症因子和细胞凋亡通路参与疾病进展，抗体用于Western blot和免疫组化验证AhR蛋白水平变化。
2. **"Development and characterization of a novel monoclonal antibody against the aryl hydrocarbon receptor"**
*作者：Hidaka T, et al.*
**摘要**：报道了一种新型AhR单克隆抗体的开发与验证，该抗体在免疫沉淀和流式细胞术中表现出高特异性，成功应用于AhR蛋白的亚细胞定位及与配体结合后的构象变化研究。
3. **"Aryl hydrocarbon receptor deficiency attenuates oxidative stress-related mesangial cell activation"**
*作者：Itoh K, et al.*
**摘要**：通过AhR抗体(ChIP实验)证实AhR缺失可减少氧化应激诱导的肾小球系膜细胞活化，揭示AhR在调控Nrf2通路中的关键作用，抗体用于染色质免疫共沉淀分析AhR与靶基因启动子的结合。
4. **"The aryl hydrocarbon receptor interacts with c-Maf to promote the differentiation of type 1 regulatory T cells"**
*作者：Gandhi R, et al.*
**摘要**：研究使用AhR抗体(免疫荧光共定位)揭示AhR与转录因子c-Maf相互作用，调控调节性T细胞分化，抗体用于共聚焦显微镜观察AhR在T细胞中的亚细胞分布及共定位关系。
提示：以上文献摘要基于AhR抗体的应用场景(如Western blot、免疫组化、ChIP等)和功能研究(炎症、免疫调控)，建议通过PubMed或期刊数据库搜索具体标题获取全文。
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor belonging to the basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) family. It plays a critical role in mediating cellular responses to environmental toxins, such as dioxins and polycyclic aromatic hydrocarbons, by regulating genes involved in xenobiotic metabolism (e.g., CYP1A1). Beyond its classical role in detoxification, AhR is increasingly recognized as a modulator of immune responses, cell differentiation, and homeostasis, interacting with pathways like NF-κB and STAT.
AhR antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection of AhR in techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into its activation mechanisms, nuclear translocation, and dimerization with partners like ARNT. These antibodies are widely used in toxicology, immunology, and cancer research, particularly given AhR's dual role in promoting or suppressing tumors depending on context.
Commercial AhR antibodies are often validated for specificity across species (human, mouse, rat) and applications. However, challenges remain due to AhR's structural complexity, splice variants, and cross-reactivity with related proteins. Recent studies also explore AhR's involvement in autoimmune diseases, gut immunity, and microbiome interactions, expanding the utility of these antibodies in translational research. Proper controls (e.g., knockout validation) are critical to ensure data reliability.
×
