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Human Telomerase reverse transcriptase(TERT) ELISA kit

  • 中文名： 人端粒酶逆转录酶(TERT) ELISA kit
货号: IPD-EL023391HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人端粒酶逆转录酶(TERT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TERT含量。TERT是端粒酶逆转录酶，它是端粒酶的催化亚基。其背景在于端粒酶与细胞的老化、癌变等密切相关。研究机制上，TERT可延长端粒长度，维持染色体稳定。在肿瘤研究中，它被视为潜在治疗靶点，通过抑制其活性有望控制肿瘤细胞的增殖。试剂盒检测范围为12.5 ng/ml- 800 ng/ml，该产品适用于体外研究TERT在肿瘤生物学、衰老机制、再生医学等领域的表达调控，为探索其在细胞增殖、分化及疾病模型中的作用提供高灵敏度的检测工具。实验操作兼容常规酶标仪，配套标准品及缓冲液可确保检测结果稳定，满足科研场景下对微量样本的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CMM9 ELISA Kit; DKCA2 ELISA Kit; DKCB4 ELISA Kit; EST2 ELISA Kit; HEST2 ELISA Kit; htert ELISA Kit; hTRT ELISA Kit; PFBMFT1 ELISA Kit; TCS1 ELISA Kit; Telomerase associated protein 2 ELISA Kit; Telomerase catalytic subunit ELISA Kit; Telomerase reverse transcriptase ELISA Kit; Telomerase-associated protein 2 ELISA Kit; Telomere Reverse Transcriptase ELISA Kit; TERT ELISA Kit; TERT_HUMAN ELISA Kit; TP2 ELISA Kit; TRT ELISA Kit
  • 缩写：
    TERT
  • Uniprot No.：
    O14746
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/ml - 800 ng/ml
  • 灵敏度：
    3.125 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TERT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    88-101


    1:2

    Average %

    97


    Range %

    91-102


    1:4

    Average %

    91


    Range %

    86-96


    1:8

    Average %

    93


    Range %

    87-98


  • 回收率：

    The recovery of human TERT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    88-101


    EDTA plasma (n=4)

    87

    82-94


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.886

    2.734

    2.810

    2.681


    400

    1.916

    1.867

    1.892

    1.763


    200

    1.059

    1.028

    1.043

    0.915


    100

    0.597

    0.629

    0.613

    0.484


    50

    0.368

    0.381

    0.374

    0.246


    25

    0.289

    0.293

    0.291

    0.162


    12.5

    0.196

    0.184

    0.190

    0.061


    0

    0.132

    0.125

    0.129



  • 本试剂盒所含材料：



    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TERT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled TERT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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