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产品描述：犬胸腺活化调节趋化因子(TARC/CCL17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TARC/CCL17含量。TARC/CCL17是重要的靶点。它是一种趋化因子，在免疫系统中发挥关键作用。其异常表达与多种疾病如特应性皮炎、哮喘等相关。研究机制主要聚焦于其与受体结合，介导免疫细胞迁移、活化，进而影响疾病的发生发展，为相关疾病治疗提供潜在方向。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品可为犬类过敏性皮炎模型研究、呼吸道炎症机制探索、免疫调节药物开发等科研方向提供可靠数据支持，尤其适用于动态监测疾病进程中趋化因子表达变化或评估实验性治疗干预效果，为深入解析犬类免疫调控网络提供技术工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCL17 ELISA Kit; TARCC-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; Small-inducible cytokine A17 ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
缩写：TARC/CCL17
Uniprot No.：Q95N01
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of canine TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-99 | ||||
| EDTA plasma (n=4) | 100 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15001.9082.0051.9571.8017501.1791.2051.1921.0363750.7340.7380.7360.580187.50.4150.4360.4260.270940.3110.3280.3200.164470.2310.2450.2380.08223.50.1980.1890.1940.03800.1550.1570.156 | ||||||
货期：3-5 working days
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