| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABH4 |
| Entrez GeneID | 54784 |
| WB Predicted band size | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human ALKBH4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ALKBH4抗体的3篇参考文献示例(内容基于假设性文献，仅供参考)：
1. **文献名称**：*ALKBH4 regulates spermatogenesis through demethylation of histones in mouse testes*
**作者**：Zhang Y, et al.
**摘要**：该研究利用ALKBH4特异性抗体，通过免疫组化技术发现ALKBH4在小鼠睾丸组织中的定位，并揭示其在精子形成过程中通过组蛋白去甲基化调控基因表达的作用。
2. **文献名称**：*Development of a polyclonal antibody against human ALKBH4 and its application in cancer cell analysis*
**作者**：Tanaka K, et al.
**摘要**：研究团队成功制备了针对人源ALKBH4的多克隆抗体，并通过Western blot和免疫荧光验证其特异性，进一步应用于多种癌细胞系中ALKBH4蛋白表达水平的检测。
3. **文献名称**：*ALKBH4 interacts with microtubule proteins and modulates cellular stress response*
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀(使用ALKBH4抗体)和质谱分析，研究发现ALKBH4与微管蛋白相互作用，并在细胞应激条件下通过调控微管稳定性影响细胞存活。
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注：上述文献为示例，实际引用时请以真实发表的论文为准，建议通过PubMed或Web of Science以“ALKBH4 antibody”为关键词检索相关研究。
ALKBH4 (Alkylated DNA Repair Protein AlkB Homolog 4) is a member of the AlkB-like family of dioxygenases, which are evolutionarily conserved enzymes involved in DNA/RNA repair and epigenetic regulation. Unlike other ALKBH family members (e.g., ALKBH5 or FTO) that primarily target RNA N6-methyladenosine (m6A) modifications, ALKBH4 is implicated in demethylating histone H2A and regulating actin methylation, thereby influencing cytoskeletal dynamics, cell division, and chromatin remodeling. It plays a critical role in maintaining genomic stability and cellular homeostasis.
ALKBH4 antibodies are essential tools for studying the protein's expression, localization, and function in biological systems. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ALKBH4 in tissues or cultured cells. Research has linked ALKBH4 dysregulation to various pathologies, including cancer, infertility, and neurodevelopmental disorders. For example, reduced ALKBH4 levels correlate with defective sperm maturation, while its overexpression in tumors may drive proliferation or metastasis by altering cytoskeletal organization or gene expression patterns.
Validating ALKBH4 antibodies for specificity and cross-reactivity is crucial due to structural similarities within the ALKBH family. High-quality antibodies enable researchers to explore ALKBH4’s substrate specificity, its interplay with other epigenetic modifiers, and its potential as a therapeutic target. Ongoing studies aim to clarify its mechanisms in development and disease, emphasizing the need for reliable detection tools.
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