| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABH2 |
| Entrez GeneID | 121642 |
| WB Predicted band size | Calculated MW: 29 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ALKBH2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4条关于ALKBH2抗体的参考文献示例(内容基于领域内典型研究方向概括，具体文献需根据实际检索调整)：
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1. **文献名称**: "ALKBH2 promotes glioblastoma progression by regulating DNA damage response"
**作者**: Smith J, et al.
**摘要**: 本研究利用ALKBH2特异性抗体，通过免疫组化(IHC)和Western blot分析，揭示了ALKBH2在胶质母细胞瘤组织中的高表达及其通过调控DNA损伤修复通路促进肿瘤生长的机制。
2. **文献名称**: "Development of a monoclonal antibody against human ALKBH2 for detection of alkylation repair activity"
**作者**: Tanaka K, et al.
**摘要**: 研究团队开发了一种高特异性ALKBH2单克隆抗体，验证了其在ELISA和免疫荧光中的有效性，并应用于检测癌细胞中ALKBH2介导的烷基化损伤修复活性。
3. **文献名称**: "ALKBH2 deficiency enhances sensitivity to cisplatin in non-small cell lung cancer"
**作者**: Wang L, et al.
**摘要**: 通过ALKBH2抗体敲低实验，结合临床样本分析，发现ALKBH2低表达与非小细胞肺癌患者对顺铂化疗敏感性增加相关，提示其作为治疗靶点的潜力。
4. **文献名称**: "The role of ALKBH2 in maintaining genome stability: Insights from antibody-based functional assays"
**作者**: Garcia R, et al.
**摘要**: 利用ALKBH2抗体进行染色质免疫沉淀(ChIP)和流式细胞术，证明ALKBH2通过修复DNA N1-甲基腺嘌呤损伤维持基因组稳定性，缺陷可能导致细胞周期异常。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台以“ALKBH2 antibody”为关键词检索获取。推荐结合抗体应用场景(如疾病模型、分子机制)进一步筛选文献。
**Background of ALKBH2 Antibody**
ALKBH2 (AlkB homolog 2) is a DNA repair enzyme belonging to the AlkB family of dioxygenases, which are conserved across prokaryotes and eukaryotes. It specifically repairs alkylation-induced DNA damage, such as 1-methyladenine (1mA) and 3-methylcytosine (3mC), through oxidative demethylation—a process critical for maintaining genomic stability. ALKBH2’s role in DNA repair has linked it to cancer biology, as dysregulation may contribute to mutagenesis or therapeutic resistance.
Antibodies targeting ALKBH2 are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in the exploration of ALKBH2’s tissue-specific distribution and subcellular dynamics (primarily nuclear). Such antibodies also facilitate research into its interactions with DNA repair pathways or regulatory proteins.
In cancer research, ALKBH2 antibodies help assess its potential as a biomarker or therapeutic target, particularly in tumors with alkylation damage vulnerabilities (e.g., gliomas). Additionally, these antibodies support investigations into ALKBH2’s role beyond DNA repair, including possible involvement in epigenetic regulation or RNA modification. Validation of antibody specificity—through knockout controls or enzymatic activity assays—is critical to ensure reliable experimental outcomes. Overall, ALKBH2 antibodies are vital for advancing insights into genome integrity mechanisms and disease-related dysfunctions.
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