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产品描述：人转胶蛋白2(TAGLN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物样本中的TAGLN2含量。TAGLN2是一种与肌动蛋白结合的蛋白，被认为具有肿瘤抑制功能。其研究机制涉及调节T细胞脂质代谢，通过促进脂肪酸结合蛋白5（FABP5）的运输至T细胞表面，增强T细胞在肿瘤微环境中的抗肿瘤功能。TAGLN2的表达受肿瘤微环境中的内质网应激反应调节，其过表达可能有助于提高癌症免疫疗法的疗效。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可利用开展疾病相关分子机制研究，例如通过检测不同处理条件下细胞裂解液或体液样本中TAGLN2的动态变化，探索其在细胞运动、炎症反应或代谢调控中的功能，也可用于药物开发过程中靶点蛋白表达水平的定量评估。本产品适用于生命科学基础研究与转化医学领域的蛋白定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CDABP0035 ELISA Kit; epididymis tissue protein Li 7e ELISA Kit; HA1756 ELISA Kit; KIAA0120 ELISA Kit; SM22-alpha homolog ELISA Kit; TAGL2_HUMAN ELISA Kit; Tagln2 ELISA Kit; Transgelin-2 ELISA Kit
缩写：TAGLN2
Uniprot No.：P37802
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-92 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 98-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-102 | |||||
回收率：
| The recovery of human TAGLN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3982.3672.3832.228101.6721.5761.6241.46951.0711.0621.0670.9122.50.6240.6180.6210.4661.250.4270.4110.4190.2640.6250.3190.3060.3130.1580.3120.2510.2400.2460.09100.1560.1540.155 | ||||||
货期：3-5 working days
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