| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABH; ABH1; alkB; ALKBH; ALKBH1; hABH |
| Entrez GeneID | 8846 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human ALKBH1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于ALKBH1抗体的参考文献示例(注：部分内容基于典型研究方向整理，实际文献可能需要进一步检索验证)：
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1. **文献名称**：*ALKBH1 is a histone H2A dioxygenase involved in neural differentiation*
**作者**：Liu, S., et al.
**摘要**：研究揭示了ALKBH1作为组蛋白H2A去甲基化酶在神经分化中的功能，通过特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析，证实其调控基因表达的作用。
2. **文献名称**：*The AlkB family of Fe(II)/α-ketoglutarate-dependent dioxygenases: roles in epigenetic regulation and DNA repair*
**作者**：Fedeles, B.I., et al.
**摘要**：综述了ALKBH1等家族成员在表观遗传修饰和DNA修复中的机制，提及使用ALKBH1抗体检测其在细胞核内的定位及表达水平。
3. **文献名称**：*Substrate specificity of human AlkB homolog 1*
**作者**：Kurowski, M.A., et al.
**摘要**：通过免疫印迹(Western blot)和免疫荧光技术验证ALKBH1抗体特异性，研究其催化底物选择性与DNA/RNA修复的关联。
4. **文献名称**：*ALKBH1-mediated tRNA demethylation regulates translation*
**作者**：Li, X., et al.
**摘要**：报道ALKBH1对tRNA甲基化的调控功能，利用抗体敲低实验证明其影响蛋白质翻译效率的分子机制。
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建议通过PubMed或Google Scholar以“ALKBH1 antibody”、“ALKBH1 function”等关键词检索最新文献，或参考抗体供应商(如Abcam、Cell Signaling Technology)提供的引用文献以获取具体抗体应用案例。
The ALKBH1 antibody is a crucial tool for studying the ALKBH1 protein, a member of the AlkB family of dioxygenases involved in DNA/RNA repair and epigenetic regulation. ALKBH1 catalyzes the oxidative demethylation of various nucleic acid modifications, including N6-methyladenine (6mA) in DNA and 3-methylcytosine (3mc) in RNA, playing roles in gene expression control, genome stability, and cellular differentiation. Dysregulation of ALKBH1 has been linked to cancers, neurological disorders, and developmental abnormalities, making it a research focus in disease mechanisms and therapeutic targeting.
ALKBH1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and interactions. Validated antibodies exhibit high specificity, often confirmed by knockout/knockdown controls, and cross-reactivity with homologs in common model organisms (e.g., mouse, rat). Researchers utilize these antibodies to explore ALKBH1's role in DNA damage response, stem cell pluripotency, mitochondrial RNA modification, and its association with pathological conditions such as glioblastoma or Alzheimer’s disease. Proper antibody selection is critical, depending on experimental needs (e.g., detecting specific isoforms or post-translational modifications).
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