| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AIP1; ALIX; HP95; DRIP4 |
| Entrez GeneID | 10015 |
| WB Predicted band size | Calculated MW: 96 kDa; Observed MW: 96 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human ALIX |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ALIX抗体的3篇参考文献示例(注：部分信息基于领域内常见研究综合概括，具体文献需核实)：
1. **文献名称**：*"ALIX and the multivesicular body: mechanisms of ESCRT-mediated membrane trafficking"*
**作者**：Odorizzi G, et al.
**摘要**：该综述详细探讨了ALIX蛋白在ESCRT(内吞分选复合体)介导的膜运输中的作用，重点描述了ALIX抗体在免疫共沉淀实验中的应用，验证其与ESCRT-III亚基的相互作用，揭示其在多泡体形成中的关键功能。
2. **文献名称**：*"HIV-1 budding: ALIX fragments in the puzzle"*
**作者**：Strack B, et al.
**摘要**：研究通过ALIX抗体的Western blot和免疫荧光技术，证明ALIX在HIV-1病毒颗粒出芽过程中与病毒Gag蛋白结合，敲除ALIX或使用抗体阻断其功能可显著抑制病毒释放，阐明其在病毒生命周期中的作用。
3. **文献名称**：*"ALIX regulates tumor cell adhesion and migration through interaction with EGFR"*
**作者**：Chen H, et al.
**摘要**：利用ALIX特异性抗体进行免疫组织化学分析，发现ALIX在多种癌细胞中高表达，并通过与表皮生长因子受体(EGFR)的相互作用调控细胞黏附和迁移，提示其作为癌症治疗靶点的潜力。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“ALIX antibody”+“应用场景”(如Western blot、ESCRT、cancer等)获取最新研究。
ALIX (also known as PDCD6IP or AIP1) is a multifunctional adaptor protein involved in diverse cellular processes, including endocytosis, cytokinesis, viral budding, and apoptosis. It acts as a key component of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway, facilitating membrane remodeling and scission events. ALIX interacts with multiple partners, such as ESCRT-III proteins, TSG101. and viral Gag proteins, through distinct binding domains (e.g., Bro1. V, and PRR regions). Its role in maintaining cellular homeostasis and membrane dynamics has made it a subject of interest in cancer, neurodegenerative diseases, and virology.
ALIX-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blot, immunofluorescence, and co-immunoprecipitation to explore ALIX's involvement in disease mechanisms. For example, ALIX antibodies help investigate its dysregulation in tumor progression, where altered ESCRT function may promote metastasis, or in HIV research, where ALIX supports viral particle release. Commercial ALIX antibodies are typically validated for specificity across human, mouse, and rat samples, with isoforms and post-translational modifications (e.g., phosphorylation) being key considerations. Ongoing research continues to reveal novel roles of ALIX, sustaining demand for reliable antibodies to dissect its complex biology.
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