首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胶转蛋白(TAGLN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TAGLN含量。人胶转蛋白(TAGLN)是一种肌动蛋白结合蛋白，属于钙调蛋白家族，广泛表达于血管和内脏平滑肌，是平滑肌分化的早期标志。TAGLN在肿瘤中作为肿瘤抑制基因，其低表达与多种癌症的发生发展相关，特别是在结肠癌中，其高表达可能促进肿瘤转移。研究TAGLN在结肠癌中的作用机制，有助于为预防和控制结肠癌转移提供新的分子基础和靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研人员可利用该产品探究心血管疾病、器官纤维化或肿瘤微环境中TAGLN的表达调控机制，也可用于体外细胞模型或动物实验的组织样本分析，为相关分子机制研究提供可靠数据支持。试剂盒兼容常规酶标仪检测，适用于生命科学基础研究与转化医学领域的蛋白定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TAGLN ELISA Kit; SM22 ELISA Kit; WS3-10 ELISA Kit; Transgelin ELISA Kit; 22 kDa actin-binding protein ELISA Kit; Protein WS3-10 ELISA Kit; Smooth muscle protein 22-alpha ELISA Kit; SM22-alpha ELISA Kit
缩写：TAGLN
Uniprot No.：Q01995
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TAGLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of human TAGLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-90 | ||||
| EDTA plasma (n=4) | 99 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8232.9022.8632.754102.1662.3552.2612.15251.4431.4961.4701.3612.51.0351.0521.0440.9351.250.5580.5960.5770.4680.6250.3740.3750.3750.2660.3120.2310.2480.2400.13100.1080.1090.109 | ||||||
货期：3-5 working days
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