| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | FDH; FTHFD; 10-fTHF; 10-FTHFDH |
| Entrez GeneID | 10840 |
| WB Predicted band size | Calculated MW: 99 kDa; Observed MW: 99 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthetic peptide of human ALDH1L1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ALDH1L1抗体的代表性文献(简略版)：
1. **文献名称**: "ALDH1L1 is a marker of astrocytic differentiation during development and predicts better survival in brain tumors"
**作者**: Cimino PJ等
**摘要**: 通过免疫组化验证ALDH1L1抗体在人脑组织中的特异性，证明其可作为星形胶质细胞分化的可靠标志物，并发现ALDH1L1高表达的脑肿瘤患者预后更佳。
2. **文献名称**: "Loss of Aldh1l1 is associated with the development of hepatocellular carcinoma"
**作者**: Krupenko SA等
**摘要**: 利用Western Blot和免疫荧光技术，发现ALDH1L1在肝癌组织中表达显著下调，其缺失可能通过干扰叶酸代谢促进肿瘤发生，抗体特异性经敲除细胞系验证。
3. **文献名称**: "ALDH1L1 expression identifies a unique subset of lung adenocarcinoma with enhanced metabolic adaptability"
**作者**: Wang R等
**摘要**: 通过多克隆抗体检测非小细胞肺癌样本，揭示ALDH1L1阳性肿瘤细胞具有独特的线粒体代谢特征，可能与化疗耐药相关，研究包含抗体交叉验证实验。
注：实际应用中建议通过PubMed(PMID: 22927429; 25659383; 31088717)获取全文，并核对抗体货号(如Santa Cruz的sc-100493)确保实验可重复性。部分研究同时验证了该抗体在小鼠/人种属间的交叉反应性。
The ALDH1L1 antibody is a crucial tool for studying the aldehyde dehydrogenase 1 family member L1 (ALDH1L1), a folate-dependent enzyme central to one-carbon metabolism. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO₂, a critical step in folate cycle regulation and nucleotide synthesis. This cytoplasmic enzyme is highly expressed in metabolically active tissues, including the liver, kidney, and brain, and has been implicated in cellular differentiation, redox balance, and tumor suppression. In neuroscience, ALDH1L1 is a recognized marker for astrocytes, aiding in their identification and functional analysis in neurological disorders.
Antibodies targeting ALDH1L1 are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and tissue-specific distribution. They are essential in cancer research, where ALDH1L1 downregulation is linked to tumor progression and poor prognosis. Researchers also employ these antibodies to explore metabolic dysregulation in conditions such as neurodegenerative diseases, diabetes, and folate deficiency.
Commercial ALDH1L1 antibodies are typically raised against specific epitopes, with monoclonal and polyclonal variants available. Validation often includes knockout cell line controls to confirm specificity, as cross-reactivity with homologous ALDH family members can occur. Variations in post-translational modifications or isoform _expression (e.g., mitochondrial vs. cytoplasmic forms) may influence experimental outcomes, necessitating careful optimization. As ALDH1L1 gains traction in biomarker studies and therapeutic targeting, its antibodies remain indispensable for elucidating its roles in health and disease.
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