首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠P物质受体(SP-R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的TACR1含量。TACR1是一种重要靶点。其背景上，它与神经系统等多种生理过程相关。研究机制方面，主要聚焦于其与神经肽的相互作用，通过介导信号传导影响细胞功能，针对它开发药物有望治疗疼痛、炎症等多种疾病。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本试剂盒通过特异性识别小鼠SP - R的抗体对，结合优化的反应体系，可实现复杂生物样本中目标蛋白的高效捕获与信号放大，为神经生物学、免疫调控机制研究及药物开发中的靶点验证提供可靠工具，尤其适用于动物模型中的神经信号通路探索、炎症相关分子机制解析及药效学评价等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tacr1; Tac1r; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1
缩写：TACR1
Uniprot No.：P30548
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 93-106 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-99 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-98 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 96-110 | ||||||
回收率：
| The recovery of mouse SP-R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 81-95 | |||||
| EDTA plasma (n=4) | 101 | 92-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.2262.1872.2072.042 2501.5621.5191.5411.376 1251.0061.0441.0250.860 62.50.6610.6390.6500.485 31.20.4890.5230.5060.341 15.60.4000.4150.4080.243 7.80.2740.2700.2720.107 00.1680.1620.165 | |||||||
货期：3-5 working days
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