Human Secreted phosphoprotein 2 (SPP2/SPP24) ELISA kit

货号: IPD-EL022604HU

Price： ￥2500

48T 96T

数量：

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产品描述：人分泌磷蛋白2(SPP2/SPP24)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPP2含量。试剂盒检测范围为15.6 ng/ml-1000 ng/ml，适用于体外研究SPP2/SPP24在骨骼生物学、钙磷代谢调节、组织矿化或相关疾病模型中的表达水平变化，为探索该蛋白的分子功能及病理关联提供灵敏稳定的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SPP2 ELISA Kit; SPP24 ELISA Kit; Secreted phosphoprotein 24 ELISA Kit; Spp-24 ELISA Kit; Secreted phosphoprotein 2 ELISA Kit
缩写：SPP2
Uniprot No.：Q13103
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/ml-1000 ng/ml
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SPP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	98
	Range %	96-102
1:10	Average %	107
	Range %	103-114
1:20	Average %	94
	Range %	90-98
1:40	Average %	88
	Range %	86-95

回收率：

The recovery of human SPP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected 10002.1882.2332.2112.028 5001.7321.8241.7781.595 2501.2111.2611.2361.053 1250.6980.7570.7280.545 62.50.5230.5220.5230.340 31.20.3450.3580.3520.169 15.60.2520.2600.2560.073 00.1760.1890.183　 .

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