首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人脊椎蛋白-1(SPON1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SPON1含量。SPON1（脊椎蛋白-1）是一种细胞外基质结构成分，参与细胞粘附和蛋白质结合活性的调节。其研究机制涉及在细胞外空间与胶原蛋白共定位，并可能参与淀粉样蛋白前体蛋白的分解代谢过程。在疾病如阿尔茨海默病中，SPON1的表达和功能可能发生变化，影响疾病的发病机制。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研人员可通过探究SPON1在神经退行性疾病模型中的表达特征，分析其在肿瘤微环境中的调控作用，或评估骨骼发育相关疾病中该蛋白的生物标志物价值；适用于体外实验中的基础机制研究、疾病模型分析及生物标志物筛选等科研场景，为探索SPON1的分子功能及其在病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SPON1 ELISA kit; KIAA0762 ELISA kit; VSGPSpondin-1 ELISA kit; F-spondin ELISA kit; Vascular smooth muscle cell growth-promoting factor ELISA kit
缩写：SPON1
Uniprot No.：Q9HCB6
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-101 | |||||
回收率：
| The recovery of human spondin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-104 | ||||
| EDTA plasma (n=4) | 93 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5042.4632.4842.36552.0062.0422.0241.9052.51.5061.4791.4931.3741.250.9730.8970.9350.8160.6250.5110.5180.5150.3960.3120.2850.2680.2760.1570.1560.1760.1870.1820.06300.1210.1170.119 | ||||||
货期：3-5 working days
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