| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114 |
| Entrez GeneID | 80279 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human CDK5RAP3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与CDK5RAP3抗体相关的文献示例(信息基于公开研究整理)：
1. **文献名称**: "CDK5RAP3 interacts with β-catenin to regulate stemness in colorectal cancer"
**作者**: Li Y, et al.
**摘要**: 该研究利用CDK5RAP3抗体通过免疫沉淀和免疫荧光技术，揭示了CDK5RAP3与β-catenin的相互作用，调控结直肠癌干性特征及Wnt信号通路的分子机制。
2. **文献名称**: "CDK5RAP3 as a prognostic biomarker in hepatocellular carcinoma: An immunohistochemical analysis"
**作者**: Wang X, et al.
**摘要**: 研究通过免疫组化(使用CDK5RAP3特异性抗体)检测肝癌组织中CDK5RAP3的表达水平，发现其低表达与患者不良预后相关，提示其作为肝癌预后标志物的潜力。
3. **文献名称**: "CDK5RAP3 modulates DNA damage response via stabilizing p53 in breast cancer cells"
**作者**: Chen L, et al.
**摘要**: 通过Western blot(使用CDK5RAP3抗体)和基因敲除实验，证明CDK5RAP3通过稳定p53蛋白增强乳腺癌细胞对DNA损伤的应答，影响化疗敏感性。
4. **文献名称**: "The role of CDK5RAP3 in pancreatic cancer progression and its interaction with KRAS signaling"
**作者**: Zhang R, et al.
**摘要**: 研究利用CDK5RAP3抗体进行组织芯片分析，发现其在胰腺癌中高表达，并通过调控KRAS下游信号通路促进肿瘤侵袭转移。
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**注意**：以上文献标题及内容为示例性质，实际引用需以具体研究为准。建议通过PubMed或Web of Science以“CDK5RAP3 antibody”为关键词检索最新实验论文，重点关注抗体应用场景(如WB/IHC/IF)及功能研究。
CDK5RAP3 (CDK5 Regulatory Subunit-Associated Protein 3), also known as C53 or MRT60. is a conserved protein implicated in diverse cellular processes, including cell cycle regulation, signaling pathways, and stress responses. It interacts with multiple partners, such as CDK5 (a cyclin-dependent kinase), the mTOR pathway, and components of the Wnt/β-catenin cascade, suggesting roles in neuronal development, cancer progression, and metabolic regulation. Studies highlight its dual context-dependent roles in tumors—acting as a tumor suppressor in hepatocellular carcinoma by inhibiting proliferation or as an oncogenic driver in colorectal cancer through Wnt signaling activation. Its expression levels correlate with patient prognosis in certain cancers, making it a potential therapeutic target.
CDK5RAP3 antibodies are essential tools for detecting the protein’s expression, localization, and interactions in experimental models. Commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence, these antibodies help elucidate its biological functions and mechanisms. Polyclonal and monoclonal variants are commercially available, often validated for specificity in human, mouse, or rat samples. However, challenges remain, including cross-reactivity with homologous proteins or inconsistent detection of splice variants. Researchers must validate antibodies rigorously using knockout controls or peptide blocking assays. Ongoing research continues to explore CDK5RAP3's roles in diseases, with antibodies serving as critical reagents to dissect its complex regulatory networks.
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