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产品描述：人Spondin-2酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织培养上清液样本中的SPON2含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于基础科研领域，如探究Spondin-2在肿瘤发生、神经退行性疾病或组织修复中的分子机制，也可用于体外细胞模型中蛋白分泌水平的动态监测，为疾病相关生物标志物研究及信号通路调控分析提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; DIL 1 ELISA Kit; DIL-1 ELISA Kit; DIL1 ELISA Kit; DKFZp686G21139 ELISA Kit; FLJ16313 ELISA Kit; FLJ22401 ELISA Kit; FLJ34460 ELISA Kit; M spondin ELISA Kit; Mindin ELISA Kit; ORF Names: ELISA Kit; SPON 2 ELISA Kit; Spon2 ELISA Kit; SPON2_HUMAN ELISA Kit; Spondin 2 ELISA Kit; Spondin 2 extracellular matrix protein ELISA Kit; Spondin-2 ELISA Kit
缩写：SPON2
Uniprot No.：Q9BUD6
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF), cell culture supernates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human spondin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human spondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2001.8251.8971.8611.744 1001.4141.4351.4251.308 500.9960.9810.9890.872 250.6920.6830.6880.571 12.50.4230.4580.4410.324 6.250.2950.2800.2880.171 3.120.2060.2160.2110.094 00.1160.1180.117 | ||||||
货期：3-5 working days
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