| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD63 antigen; CD63 |
| Entrez GeneID | 12512 |
| WB Predicted band size | Calculated MW: 26 kDa; Observed MW: 40-70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of mouse CD63 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于CD63抗体的3篇代表性文献及其摘要内容概括：
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1. **文献名称**: *Isolation and characterization of exosomes from cell culture supernatants and biological fluids*
**作者**: Théry, C., Amigorena, S., Raposo, G., & Clayton, A.
**摘要**: 该文献详细描述了外泌体分离和鉴定的标准方法，其中CD63抗体被广泛用作外泌体表面标志物的检测工具，通过Western blot或流式细胞术验证外泌体的存在和纯度(2006年)。
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2. **文献名称**: *Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes*
**作者**: Kowal, J., Arras, G., Colombo, M., et al.
**摘要**: 研究通过蛋白质组学分析不同细胞来源的细胞外囊泡(EVs)，发现CD63抗体能特异性识别特定EV亚群(如外泌体)，并强调其在区分EV亚型中的重要性(2016年)。
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3. **文献名称**: *Shedding light on the cell biology of extracellular vesicles*
**作者**: van Niel, G., D'Angelo, G., & Raposo, G.
**摘要**: 综述了细胞外囊泡的生物发生和功能，指出CD63作为四跨膜蛋白家族成员，其抗体常用于追踪内体来源的囊泡(如外泌体)，并探讨CD63在细胞间通讯和疾病中的作用(2018年)。
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4. **文献名称**: *Rab27a and Rab27b control different steps of the exosome secretion pathway*
**作者**: Ostrowski, M., Carmo, N.B., Krumeich, S., et al.
**摘要**: 研究揭示了Rab27蛋白调控外泌体分泌的机制，利用CD63抗体标记外泌体，证实其在分泌过程中的动态定位，为外泌体功能研究提供关键实验依据(2010年)。
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这些文献均涉及CD63抗体在细胞外囊泡(尤其是外泌体)研究中的关键应用，涵盖检测、分选和功能机制探索。
CD63 antibody is a crucial tool in studying tetraspanin proteins, which are involved in membrane organization and cellular communication. CD63. a member of the transmembrane 4 superfamily (TM4SF), is ubiquitously expressed in various cell types and predominantly localizes to late endosomes, lysosomes, and the cell membrane. It is highly enriched on exosomes and other extracellular vesicles (EVs), making it a widely used marker for EV characterization. CD63 participates in diverse cellular processes, including cell adhesion, motility, signal transduction, and intracellular trafficking. Its interactions with integrins, receptors, and other tetraspanins modulate immune responses, cancer metastasis, and viral infection pathways.
CD63 antibodies are essential for detecting and quantifying EVs in biological fluids, aiding research on their roles in cancer progression, neurodegenerative diseases, and immune regulation. These antibodies are employed in techniques like flow cytometry, Western blotting, immunofluorescence, and ELISA. Specific CD63 clones (e.g., H5C6. MEM-259) recognize distinct extracellular epitopes, which may influence experimental outcomes depending on protein conformation or post-translational modifications. Recent studies also explore CD63 as a potential therapeutic target, particularly in drug delivery systems leveraging exosomes. However, variability in antibody performance across applications necessitates careful validation to ensure specificity and reproducibility.
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