首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人骨粘连蛋白(ON)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、唾液、组织匀浆样本中的SPOCK2含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于科研场景下探究骨发育、心血管钙化、肿瘤转移等生物学过程中ON蛋白的动态变化，尤其适合多类型生物样本的批量检测需求，满足实验室对骨基质相关蛋白的高通量定量分析要求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
缩写：SPOCK2
Uniprot No.：Q92563
种属：Homo sapiens (Human)
样本类型：serum, plasma, saliva, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.6582.8572.7582.663252.3072.2082.2582.16312.51.6011.5431.5721.4776.250.8470.8670.8570.7623.120.4850.4740.4800.3851.560.3070.3190.3130.2180.780.1810.1880.1850.09000.0940.0950.095 | ||||||
货期：3-5 working days
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